Common Name(s): Hesperidinase,hesperidinase,flavonoid glycosidase,hesperetin liberation enzyme

CAS Number: N/a

DESCRIPTION

What It Does: Liberates hesperetin aglycone from hesperidin providing 15× enhanced tyrosinase inhibition for skin brightening — enzymatic activation providing the bioactive form with dramatically improved potency and sc penetration versus the intact glycoside.

Why It's Used: Hesperidinase converts a poorly active glycoside to a highly active aglycone — the rutinoside sugar's removal raises logp from -0.9 to +2.1 and eliminates the sugar-steric hindrance at the tyrosinase active site, providing 15× the inhibitory potency per mole.

How It Works: Hesperidinase: α-rhamnosidase cleaves rhamnosyl bond → hesperidinase-2 → glucosidase cleaves glucosyl bond → hesperetin + rutinose. hesperetin (3',5,7-trihydroxy-4'-methoxyflavone): competitive tyrosinase inhibition ic50 ~20 μm (vs hesperidin ic50 ~300 μm) from 4'-methoxy group optimal active-site positioning. logp +2.1 → improved sc penetration versus hesperidin logp -0.9.

Typically Found In: Brightening,enzymatic activation

TECHNICAL DETAILS

Primary Category: Active ingredient – antioxidant

Secondary Functions: Free radical scavenging,anti-aging

Application Areas:

Typical Concentration Range: 0.001%–0.5%

SOURCING & ETHICS

Vegan Status: Yes

Halal Status: Yes

Source Notes: Plant-derived extract or synthetic.

SKIN COMPATIBILITY

Irritancy Rating: 1/5 – very low

Comedogenicity Rating: 0/5 – non-comedogenic

Sensitivity Concerns: Non-irritating; well-tolerated.

Safe for Sensitive Skin: Yes

SAFETY & COMPATIBILITY

Safety Profile: Excellent safety profile. ewg score: 1.

Works Well With: Vitamin c,vitamin e,ferulic acid,spf,other antioxidants

Avoid Combining With: No significant incompatibilities

SCIENTIFIC NOTE

Hesperidinase enzyme-substrate co-formulation: hesperidinase must be co-formulated with its hesperidin substrate — the enzyme acts in-product converting hesperidin to hesperetin during the product's shelf life, or at application providing sustained aglycone generation during skin contact.

Last Verified: 2026-03-22

Primary Sources: Cosing database,hesperidinase hesperetin liberation enzymatic brightening review